Universität Tübingen Botanisches Institut
Lehrstuhl Physiologische Ökologie der Pflanzen
Nehls U, Wiese J, Hampp R (2000) Cloning of a Picea abies monosaccharide transporter gene and expression-analysis in plant tissues and ectomycorrhizas. Trees (in press) Ectomycorrhizas are formed between certain soil fungi and fine roots of woody plants. An important feature of this symbiosis is the supply of photoassimilates to the fungus. Hexoses, formed from sucrose in the common apoplast at the root/fungus interface, can be taken up by both plant and fungal monosaccharide transporters. Recently we characterized a monosaccharide transporter from the ectomycorrhizal fungus Amanita muscaria. This transporter was up-regulated in mycorrhizas, thus increasing the hexose uptake capacity of the fungal partner in symbiosis. In order to characterize host (P. abies) root monosacharide transporters, degenerate oligonucleotide primers, designed to match conserved regions from known plant hexose transporters, were used to isolate a cDNA fragment of a transporter by PCR. This fragment was used to identify a presumable full length clone (PaMST1) in a P. abies/A. muscaria mycorrhizal cDNA library. The entire cDNA codes for an open reading frame of 513 amino acids, revealing best homology to H+/monosaccharide transporters from Arabidopsis, Saccharum and Ricinus. PaMST1 was highly expressed in the hypocotyl and in roots of P. abies seedlings, but not in needles. Mycorrhiza formation lead to a slight reduction of PaMST1 expression. The results are discussed with special reference to carbon allocation in ectomycorrhizas.
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