LOCUS       pUBC-EOS-DEST       11514 bp    DNA     circular     30-SEP-2010
SOURCE      
  ORGANISM  
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Grefen,C., Donald,N., Hashimoto,K., Kudla,J., Schumacher,K. and
            Blatt,M.R.
  TITLE     A ubiquitin-10 promoter-based vector set for fluorescent protein
            tagging facilitates temporal stability and native protein
            distribution in transient and stable expression studies
  JOURNAL   Plant J. 64 (2), 355-365 (2010)
   PUBMED   20735773
FEATURES             Location/Qualifiers
     CDS             901..1560
                     /label=CmR
     misc_recomb     668..792
                     /label=attR1
     misc_recomb     complement(2248..2372)
                     /label=attR2
     CDS             1902..2207
                     /label=ccdB
     misc_feature    complement(4557..4889)
                     /label=LB
     misc_feature    11074..11222
                     /label=RB
     terminator      3145..3353
                     /label=T35S
     CDS             3403..4553
                     /label=BarR
     promoter        1..639
                     /label=pUBQ10
     CDS             4953..5963
                     /label=SpecR
     exon            2434..3105
                     /label=EosFP
     rep_origin      7504..10097
                     /label=pVS1
     rep_origin      6414..7094
                     /label=pBR322
 
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt 
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa 
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt 
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa 
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa 
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct 
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat 
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt 
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat 
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat 
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctaagg 
      661 gcccatcaca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa 
      721 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat 
      781 ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg 
      841 tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa 
      901 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa 
      961 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat 
     1021 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt 
     1081 cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt 
     1141 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa 
     1201 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat 
     1261 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag 
     1321 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg 
     1381 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc 
     1441 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat 
     1501 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa 
     1561 acgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt 
     1621 tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtatgcta 
     1681 tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata 
     1741 tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg 
     1801 tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga 
     1861 aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt aaggtttaca 
     1921 cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca 
     1981 cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct 
     2041 cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg 
     2101 atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg 
     2161 aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc 
     2221 ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat 
     2281 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac 
     2341 gtttctcgtt cagctttctt gtacaaagtg gtgatgggac gtccgcggag atctacgcgt 
     2401 gtcgactcga gatatccaac tagtgtcgcc accatgagtg cgattaagcc agacatgaag 
     2461 atcaacctcc gtatggaagg caacgtaaac gggcaccact ttgtgatcga cggagatggt 
     2521 acaggcaagc cttttgaggg aaaacagagt atggatcttg aagtcaaaga gggcggacct 
     2581 ctgccttttg cctttgatat cctgaccact gcattccatt acggcaacag ggtattcgcc 
     2641 gaatatccag accacataca agactatttt aagcagtcgt ttcctaaggg gtattcgtgg 
     2701 gaacgaagct tgactttcga agacgggggc atttgcattg ccagaaacga cataacaatg 
     2761 gaaggggaca ctttctataa taaagttcga tttcacggtg taaactttcc cgccaatggt 
     2821 ccagttatgc agaagaagac gctgaaatgg gagccctcca ctgagaaaat gtatgtgcgt 
     2881 gatggagtgc tgactggtga tattcgcatg gctttgttgc ttgaaggaaa tgcccattac 
     2941 cgatgtgact tcagaactac ttacaaagct aaggagaagg gtgtcaagtt accaggctac 
     3001 cactttgtgg accactgcat tgagatttta agccatgaca aagattacaa caaggttaag 
     3061 ctgtatgagc atgctgttgc tcattctgga ttgcctgaca atgccagacg ataagcggcc 
     3121 ttataagcgg ccatgctaga gtccgcaaaa atcaccagtc tctctctaca aatctatctc 
     3181 tctctatttt tctccagaat aatgtgtgag tagttcccag ataagggaat tagggttctt 
     3241 atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta 
     3301 aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtga cctgcaggca 
     3361 tgcgacgtcg ggccctctag aggatccccg ggtaccgcga attatcgatc atgagcggag 
     3421 aattaaggga gtcacgttat gacccccgcc gatgacgcgg gacaagccgt tttacgtttg 
     3481 gaactgacag aaccgcaacg ttgaaggagc cactgagccg cgggtttctg gagtttaatg 
     3541 agctaagcac atacgtcaga aaccattatt gcgcgttcaa aagtcgccta aggtcactat 
     3601 cagctagcaa atatttcttg tcaaaaatgc tccactgacg ttccataaat tcccctcggt 
     3661 atccaattag agtctcatat tcactctcaa ctcgatcgag gggatctacc atgagcccag 
     3721 aacgacgccc ggccgacatc cgccgtgcca ccgaggcgga catgccggcg gtctgcacca 
     3781 tcgtcaacca ctacatcgag acaagcacgg tcaacttccg taccgagccg caggaaccgc 
     3841 aggagtggac ggacgacctc gtccgtctgc gggagcgcta tccctggctc gtcgccgagg 
     3901 tggacggcga ggtcgccggc atcgcctacg cgggtccctg gaaggcacgc aacgcctacg 
     3961 actggacggc cgagtcgacc gtgtacgtct ccccccgcca ccagcggacg ggactgggct 
     4021 ccacgctcta cacccacctg ctgaagtccc tggaggcaca gggcttcaag agcgtggtcg 
     4081 ctgtcatcgg gctgcccaac gacccgagcg tgcgcatgca cgaggcgctc ggatatgccc 
     4141 cccgcggcat gctgcgggcg gccggcttca agcacgggaa ctggcatgac gtgggtttct 
     4201 ggcagctgga cttcagcctg ccggtgccgc cccgtccggt cctgcccgtc accgaaatct 
     4261 gatgacccct agagtcaagc agatcgttca aacatttggc aataaagttt cttaagattg 
     4321 aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat 
     4381 gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc 
     4441 ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa 
     4501 ttatcgcgcg cggtgtcatc tatgttacta gatcgaccgg catgcaagct gataattcaa 
     4561 ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 
     4621 caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 
     4681 gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 
     4741 gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 
     4801 aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 
     4861 cgatgacaga gcgttgctgc ctgtgatcaa ttcgggcacg aacccagtgg acataagcct 
     4921 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct 
     4981 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt 
     5041 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc 
     5101 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa 
     5161 agttaaacat catgggggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag 
     5221 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg 
     5281 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa 
     5341 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc 
     5401 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca 
     5461 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca 
     5521 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa 
     5581 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg 
     5641 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc 
     5701 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg 
     5761 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc 
     5821 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag 
     5881 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga 
     5941 tcaccaaggt agtcggcaaa taatgtctag ctagaaattc gttcaagccg acgccgcttc 
     6001 gcggcgcggc ttaactcaag tcgttagatg cactaagcac ataattgctc acagccaaac 
     6061 tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg 
     6121 ggagatatat catgcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 
     6181 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 
     6241 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 
     6301 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt 
     6361 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 
     6421 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 
     6481 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 
     6541 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 
     6601 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 
     6661 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 
     6721 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 
     6781 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 
     6841 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 
     6901 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 
     6961 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt 
     7021 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc 
     7081 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa 
     7141 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg 
     7201 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga 
     7261 ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc 
     7321 cctggtagat tgcctggccc taggccagcc atttttgagc ggccagcggc cgcgataggc 
     7381 cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc 
     7441 tcttcggctg tgcgctggcc agacagttat gcacaggcca ggcgggtttt aagagtttta 
     7501 ataagtttta aagagtttta ggcggaaaaa tcgccttttt tctcttttat atcagtcact 
     7561 tacatgtgtg accggttccc aatgtacggc tttgggttcc caatgtacgg gttccggttc 
     7621 ccaatgtacg gctttgggtt cccaatgtac gtgctatcca caggaaagag accttttcga 
     7681 cctttttccc ctgctagggc aatttgccct agcatctgct ccgtacatta ggaaccggcg 
     7741 gatgcttcgc cctcgatcag gttgcggtag cgcatgacta ggatcgggcc agcctgcccc 
     7801 gcctcctcct tcaaatcgta ctccggcagg tcatttgacc cgatcagctt gcgcacggtg 
     7861 aaacagaact tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac 
     7921 catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg 
     7981 ccgggatcga tcaaaaagta atcggggtga accgtcagca cgtccgggtt cttgccttct 
     8041 gtgatctcgc ggtacatcca atcagctagc tcgatctcga tgtactccgg ccgcccggtt 
     8101 tcgctcttta cgatcttgta gcggctaatc aaggcttcac cctcggatac cgtcaccagg 
     8161 cggccgttct tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg 
     8221 caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca gaacttgagt 
     8281 acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat 
     8341 cggttcatgg attcggttag atgggaaacc gccatcagta ccaggtcgta atcccacaca 
     8401 ctggccatgc cggccggccc tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg 
     8461 atcacctcgc cagctcgtcg gtcacgcttc gacagacgga aaacggccac gtccatgatg 
     8521 ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg 
     8581 tcgcccttgg gcggcttcct aatcgacggc gcaccggctg ccggcggttg ccgggattct 
     8641 ttgcggattc gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg 
     8701 cgttgccgct gggcggcctg cgcggccttc aacttctcca ccaggtcatc acccagcgcc 
     8761 gcgccgattt gtaccgggcc ggatggtttg cgaccgtcac gccgattcct cgggcttggg 
     8821 ggttccagtg ccattgcagg gccggcagac aacccagccg cttacgcctg gccaaccgcc 
     8881 cgttcctcca cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat 
     8941 gccgcctcct ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg 
     9001 gtagctgcgc gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt 
     9061 acatcttcag cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg 
     9121 gcgtgtctgc caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg 
     9181 cacttagcgt gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt 
     9241 tgatttaatt tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga 
     9301 ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg 
     9361 ggactcaaga atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg 
     9421 cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc 
     9481 aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg 
     9541 ctgcaccgga atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc 
     9601 ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc 
     9661 aatcgtcggg cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca 
     9721 atcgcgggca ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg 
     9781 cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag 
     9841 tacagcgata accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata 
     9901 tacgcagcga ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg 
     9961 cggcgctcgg tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac 
    10021 aaaccggcca ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg 
    10081 tacccggccg cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg 
    10141 ccgtcctggt gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg 
    10201 cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg 
    10261 tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg 
    10321 cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct 
    10381 gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc 
    10441 gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca 
    10501 cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac 
    10561 gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc 
    10621 gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca 
    10681 tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg 
    10741 tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga 
    10801 cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt 
    10861 tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa 
    10921 agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag 
    10981 acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag 
    11041 tactttaaag tactttaaag tactttaaag tactttgatc ccgaggggaa ccctgtggtt 
    11101 ggcatgcaca tacaaatgga cgaacggata aaccttttca cgccctttta aatatccgtt 
    11161 attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga 
    11221 tagtttaaac tgaaggcggg aaacgacaat ctgatccaag ctcaagctgc tctagccaat 
    11281 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 
    11341 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 
    11401 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 
    11461 ataacaattt cacacaggaa acagctatga ccatgattac gaattcgagc tcgg 
//
