LOCUS pUBN-cYFP-DEST 11030 bp DNA circular 30-SEP-2010 SOURCE ORGANISM REFERENCE 1 (bases 1 to 10818) AUTHORS Grefen,C., Donald,N., Hashimoto,K., Kudla,J., Schumacher,K. and Blatt,M.R. TITLE A ubiquitin-10 promoter-based vector set for fluorescent protein tagging facilitates temporal stability and native protein distribution in transient and stable expression studies JOURNAL Plant J. 64 (2), 355-365 (2010) PUBMED 20735773 FEATURES Location/Qualifiers misc_feature complement(4073..4405) /label=LB misc_feature 10590..10738 /label=RB terminator 2661..2869 /label=T35S CDS 2919..4069 /label=BarR promoter 1..639 /label=pUBQ10 CDS 4469..5479 /label=SpecR misc_recomb 933..1057 /label=attR1 CDS 2166..2471 /label=ccdB misc_recomb complement(2512..2636) /label=attR2 CDS 1166..1843 /label=CmR exon 666..920 /label=cYFP rep_origin 5930..6610 /label=pBR322 rep_origin 7020..9613 /label=pVS1 BASE COUNT 2489 a 2939 c 2891 g 2711 t ORIGIN 1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt 61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa 121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt 181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa 241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa 301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct 361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat 421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt 481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat 541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat 601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagaa 661 ctagtatgga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg 721 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc 781 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca 841 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg 901 gcatggacga gctgtacaag caattgttaa tcacaagttt gtacaaaaaa gctgaacgag 961 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta 1021 cataatactg taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg 1081 ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag 1141 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga 1201 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac 1261 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa 1321 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga 1381 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta 1441 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga 1501 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc 1561 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag 1621 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac 1681 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca 1741 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg 1801 cgatgagtgg caggcggggc gtaatctaga ggatccggct tactaaaagc cagataacag 1861 tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga 1921 agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca 1981 gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat 2041 gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat 2101 ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg 2161 gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg 2221 atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg 2281 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg 2341 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag 2401 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct 2461 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac 2521 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat 2581 atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtgatt 2641 aagcggccat gctagagtcc gcaaaaatca ccagtctctc tctacaaatc tatctctctc 2701 tatttttctc cagaataatg tgtgagtagt tcccagataa gggaattagg gttcttatag 2761 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat 2821 acttctatca ataaaatttc taattcctaa aaccaaaatc cagtgacctg caggcatgcg 2881 acgtcgggcc ctctagagga tccccgggta ccgcgaatta tcgatcatga gcggagaatt 2941 aagggagtca cgttatgacc cccgccgatg acgcgggaca agccgtttta cgtttggaac 3001 tgacagaacc gcaacgttga aggagccact gagccgcggg tttctggagt ttaatgagct 3061 aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt cactatcagc 3121 tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc ctcggtatcc 3181 aattagagtc tcatattcac tctcaactcg atcgagggga tctaccatga gcccagaacg 3241 acgcccggcc gacatccgcc gtgccaccga ggcggacatg ccggcggtct gcaccatcgt 3301 caaccactac atcgagacaa gcacggtcaa cttccgtacc gagccgcagg aaccgcagga 3361 gtggacggac gacctcgtcc gtctgcggga gcgctatccc tggctcgtcg ccgaggtgga 3421 cggcgaggtc gccggcatcg cctacgcggg tccctggaag gcacgcaacg cctacgactg 3481 gacggccgag tcgaccgtgt acgtctcccc ccgccaccag cggacgggac tgggctccac 3541 gctctacacc cacctgctga agtccctgga ggcacagggc ttcaagagcg tggtcgctgt 3601 catcgggctg cccaacgacc cgagcgtgcg catgcacgag gcgctcggat atgccccccg 3661 cggcatgctg cgggcggccg gcttcaagca cgggaactgg catgacgtgg gtttctggca 3721 gctggacttc agcctgccgg tgccgccccg tccggtcctg cccgtcaccg aaatctgatg 3781 acccctagag tcaagcagat cgttcaaaca tttggcaata aagtttctta agattgaatc 3841 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa 3901 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc 3961 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat 4021 cgcgcgcggt gtcatctatg ttactagatc gaccggcatg caagctgata attcaattcg 4081 gcgttaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat 4141 ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc 4201 ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga 4261 gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact 4321 aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat 4381 gacagagcgt tgctgcctgt gatcaattcg ggcacgaacc cagtggacat aagcctgttc 4441 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac 4501 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt 4561 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg 4621 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt 4681 aaacatcatg ggggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg 4741 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt 4801 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct 4861 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg 4921 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc 4981 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct 5041 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc 5101 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc 5161 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga 5221 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt 5281 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc 5341 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg 5401 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac 5461 caaggtagtc ggcaaataat gtctagctag aaattcgttc aagccgacgc cgcttcgcgg 5521 cgcggcttaa ctcaagtcgt tagatgcact aagcacataa ttgctcacag ccaaactatc 5581 aggtcaagtc tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag 5641 atatatcatg catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 5701 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 5761 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 5821 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag 5881 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 5941 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 6001 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 6061 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 6121 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 6181 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 6241 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 6301 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 6361 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 6421 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 6481 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt 6541 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt 6601 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc 6661 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac 6721 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca 6781 gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg 6841 gtagattgcc tggccctagg ccagccattt ttgagcggcc agcggccgcg ataggccgac 6901 gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt 6961 cggctgtgcg ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa 7021 gttttaaaga gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca 7081 tgtgtgaccg gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa 7141 tgtacggctt tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt 7201 tttcccctgc tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg 7261 cttcgccctc gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct 7321 cctccttcaa atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac 7381 agaacttctt gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc 7441 tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg 7501 gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga 7561 tctcgcggta catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc 7621 tctttacgat cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc 7681 cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg 7741 tttctaccag gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt 7801 ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt 7861 tcatggattc ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg 7921 ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca 7981 cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc 8041 gactatcgcg ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc 8101 ccttgggcgg cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc 8161 ggattcgatc agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt 8221 gccgctgggc ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc 8281 cgatttgtac cgggccggat ggtttgcgac cgtcacgccg attcctcggg cttgggggtt 8341 ccagtgccat tgcagggccg gcagacaacc cagccgctta cgcctggcca accgcccgtt 8401 cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg 8461 cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag 8521 ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat 8581 cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt 8641 gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact 8701 tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat 8761 ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca 8821 agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac 8881 tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg 8941 cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg 9001 cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc 9061 accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg 9121 ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc 9181 gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg 9241 cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg 9301 gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca 9361 gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg 9421 cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc 9481 gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac 9541 cggccaggat ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc 9601 cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt 9661 cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc 9721 ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac 9781 ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc 9841 cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc 9901 cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc 9961 gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt 10021 caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag 10081 gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc 10141 caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct 10201 ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca 10261 gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg 10321 ggcatagccc agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta 10381 gtcgcaagta ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg 10441 cagggcggca gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg 10501 ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact 10561 ttaaagtact ttaaagtact ttaaagtact ttgatcccga ggggaaccct gtggttggca 10621 tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgttattc 10681 taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt 10741 ttaaactgaa ggcgggaaac gacaatctga tccaagctca agctgctcta gccaatacgc 10801 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 10861 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 10921 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 10981 caatttcaca caggaaacag ctatgaccat gattacgaat tcgagctcgg //