Services

• Library preparation (including quality control and validation)
  • Genomic libraries with different insert lengths or PCR free
  • RNA-Seq libraries
  • Whole genome/Exome-Seq libraries
• DNA/RNA sequencing using Illumina MiSeq, HiSeq 3000 and NovaSeq platforms, as well as PacBio Sequel and Oxford Nanopore sequencing.

• Sample preparation for proteomics
  • Enzymatic digest
  • Fractionation of proteins (1D-PAGE) and peptides (isoelectric focussing (IEF))
• Quantitative shotgun proteomics assays
  • Stable isotope labeling of aminoc acids in cell culture (SILAC)
  • Label-free quantification
• LC-MS/MS measurements on high resolution platforms
  • Thermo Fisher LTQ-Orbitrap XL
  • Thermo Fisher LTQ-Orbitrap Elite
  • Thermo Fisher Q-Exactive plus
• Quantitative targeted proteomics
  • Targeted MS2 assays using Thermo Fisher q-Exactive plus
• Analysis of postranslational modifications (PTM)
• Routine analysis of phosporylated proteins via TiO2 enrichment of peptides

• Metabolomics profiling of metabolites (untargeted Metabolomics)
  • Waters Synapt G2
• Targeted metabolomics
  • Absolute quantification using Biocrates chemistry
  • Quantification using multiple reaction monitoring (MRM)

• Transfer, storage and user-authorized access to large OMICS data
  • OpenBIS is used as a central data management facility. Data moving is done using Rsync routines as part of the OpenBIS data mover
• Web-portal-based data access to data and analysis pipelines
  • Liferay is used as Portal solution
  • gUSE is in use a production workflow system and as an interface the high-performance-computing (HPC) cluster

• Comprehensive analysis pipelines
• Web-portal-based data visualization
• Statistical assessment and support in biological interpretation

• Small-Angle X-ray Scattering (SAXS), in collaboration with AG Schreiber (http://www.soft-matter.uni-tuebingen.de), Institute for Applied Physics, University of Tübingen.

Small-Angle X-ray Scattering is a powerful technique to study the structure and interactions of various systems with a typical size on the order of 1 nm to 100 nm. Particularly the properties of biological macromolecules such as DNA and proteins can be determined by SAXS.

The newly installed SAXS/WAXS system can be used for the following researches:

1. Structure of materials (crystalline, amorphous, or porous)
2. Solution structure of nanoparticles, colloids, surfactant, polymer or biomacromolecules (protein or DNA)
3. Kinetics of phase transition, self-assembly, etc.

For more information about SAXS/WAXS please visit: http://www.soft-matter.uni-tuebingen.de/index.html?saxs.html.